Part:BBa_K133034:Design

LPP-CF-RGD-Histop

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1291
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 848
  Illegal BamHI site found at 1359
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 385
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1642
  

Design Notes

The part was cloned into pSB1.AK3 without terminator for use in bacterial cells (surface expression).

Source

LPP was ordered from GeneArt, CF was from a plasmid, RGD-Hisstop was made with annealing.